[NEOPLASTIC HEMATOLOGICAL DISORDERS PERIPHERAL BLOOD]
Indications
Cytogenetic analysis of peripheral blood (if 10% or more blasts present) can identify numerical and structural chromosomal aberrations that are diagnostic and/or prognostic for some types of leukemia and lymphoma. Chromosome analysis is often employed for staging, monitoring treatment and predicting relapse. Leukemias and lymphomas can be distinguished by specific chromosome abnormalities which can aid in precise diagnosis, disease etiology, patient prognosis and disease management.
Collection Procedure
Green top tube (sodium heparin). Label tube with patient’s name and date of birth. A completed test requisition form must be included with every sample. A transport kit is available upon request.
Specimen Requirements
5-10 ml whole blood. Minimum 5 x 106 cell yield must contain 10% or greater, less-mature myelocytes, promyelocytes or blasts. The white blood count (WBC) is a rough guide to estimate circulating malignant cells.
Specimen Container
Sodium heparin (green top). Do not collect in lithium heparin.
Methodology
Laboratory testing for Hematological Disorders involves Tissue culture, microscopic analysis, digital image capture and karyotype production
Causes for Rejection
Frozen specimen, clotted or hemolysed, inappropriate collection tube
Transport
Ship at room temperature. Do not freeze. Do not centrifuge. Must be received within 24 hours of collection. Delay in shipping may compromise cell viability and results.
Turnaround Time
7-9 days