CHROMOSOME ANALYSIS: SOLID TUMOR

Indications

Many solid tumors, especially soft tissue sarcomas, have precise structural chromosomal abnormalities that are important for precise diagnosis.  Cancer cytogenetic studies can identify numerical and structural chromosomal abnormalities that are diagnostic and/or prognostic for various types of solid tumors. 

Collection Procedure
Aseptically transfer the specimen into sterile plastic conical tubes containing sterile transport medium.  Label tube with patient’s name and date of birth.  A completed test requisition form must be included with every sample.  A transport kit is available upon request.

Specimen Requirements

3-4 mm tumor biopsy obtained by aseptic method; larger specimens should be divided into multiple tubes of sterile transport medium (sterile transport medium available from CytoGenX).

Do not place in fixative.

  • Please Note: In the absence of CytoGenX transport tubes, use any sterile screw-top container with tissue culture medium preferably Hanks Balanced Salt Solution and add antibiotics: Penicillin/Streptomycin (100 IU/ml/100 μg/ml) or Gentamycin (50 μg/ml)

Specimen Container

Sterile screw top container containing sterile transport medium 

Methodology

Tissue culture, microscopic analysis, digital image capture and karyotype production

Causes for Rejection

Frozen specimen, clotted or hemolysed, inappropriate collection tube 

Transport

Ship at room temperature. Do not freeze.  Do not centrifuge. Must be received within 24 hours of collection. Delay in shipping may compromise cell viability and results.

Turnaround Time

8-10 days