[NEOPLASTIC HEMATOLOGICAL DISORDERS BONE MARROW]
Indications For Bone Marrow Testing
Since bone marrow is composed of actively dividing cells, and is usually the source of lymphomas and leukemic cells, it is more often the specimen of choice for analysis. Cytogenetic analysis of bone marrow biopsy can identify acquired chromosome abnormalities that are diagnostic and/or prognostic for some types of leukemia and lymphoma. Chromosome analysis is often employed for staging, monitoring treatment and predicting relapse of hematological disorders. Leukemias and lymphomas can be distinguished by specific chromosome abnormalities which can aid in precise diagnosis, disease etiology, patient prognosis and disease management.
Draw into heparinized syringe (50U/ml) to prevent clotting and transfer into green top tube (sodium heparin) for transport. Label tube with patient’s name and date of birth. A completed test requisition form must be included with every sample. A transport kit is available upon request.
2-3 ml bone marrow. (minimum 5 x 106 cells / ideally 10-20 x 106 cells)
- If bone marrow cannot be aspirated, a 1cm bone core specimen can be submitted in sterile transport medium available from CytoGenX (do not fix)
Sodium heparin (green top). Do not collect in lithium heparin.
Tissue culture, microscopic analysis, digital image capture and karyotype production
Causes for Rejection
Frozen specimen, clotted or hemolysed, inappropriate collection tube
Ship at room temperature. Do not freeze. Do not centrifuge. Must be received within 24 hours of collection. Delay in shipping may compromise cell viability and results.